other:Article Title: A 3D"Sandwich" Co-culture System with hPPSCs and hUVECs Supports Mouse Embryo Development from E3.5 to E7.5 In Vitro
Article Snippet: IVCM was composed of 25% Dulbecco's modi ed eagle medium (DMEM) with high glucose (v/v) (Adamas Life, C8013) supplemented with 75% heat-inactivated (HI) horse serum (v/v) (Gibco, 26050088), 11 mM HEPES (Solarbio, H1095), 100 × GlutaMax (Gibco, 35050-061), 100 × Penicillin Streptomycin (Gibco, 15140-122), 100 × Minimal Essential Medium (MEM) Non-essential Amino Acids (NEAA) (Gibco, 11140-050), 55 μM 2-mercaptoethanol (Gibco, 21985-023), 100 × ITS-X (Gibco, 51500-056), 8 nM β-estradiol (Sigma, HY-B0141), 200 ng/ml progesterone (Sigma, HY-N0437), 25 μM Nacetyl-L-cysteine (Sigma, HY-B0215), 0.25 × N 2 (Gibco, 17502048), 0.5 × B 27 (Gibco, 17504044).
Article Title: Loading of Extracellular Vesicles with Nucleic Acids via Hybridization with Non-Lamellar Liquid Crystalline Lipid Nanoparticles.
Article Snippet: Dulbecco’s modified eagle medium (DMEM), phenol red-free DMEM, fetal bovine serum (FBS), GlutaMAX supplement, penicillin-streptomycin (10 000 U mL−1), minimal essential medium (MEM) non-essential amino acids (NEAA) solution (100×), Opti-MEM, trypsin-EDTA (0.25%), UltraPure DNase/RNase-free distilled water, UltraPure agarose, phosphate buffered saline (PBS), Dulbecco’s phosphate buffered saline (DPBS), HEPES 1 M solution, ethanol absolute, Hoechst 33342 and blasticidin S hydrochloride (from Streptomyces Griseochromogenes) were purchased from Thermo Fisher Scientific (USA).
Article Title: Loading of extracellular vesicles with nucleic acids via hybridization with sponge-like lipid nanoparticles
Article Snippet: Dulbecco’s modified eagle medium (DMEM), phenol red-free DMEM, fetal bovine serum (FBS), GlutaMAX TM supplement, penicillin-streptomycin (10,000 U/mL), minimal essential medium (MEM) non-essential amino acids (NEAA) solution (100x), Opti-MEM TM , trypsin-EDTA (0.25%), UltraPure TM DNase/RNase-free distilled water, UltraPure TM agarose, phosphate buffered saline (PBS), Dulbecco’s phosphate buffered saline (DPBS), HEPES 1 M solution, ethanol absolute, Hoechst 33342 and blasticidin S hydrochloride (from Streptomyces Griseochromogenes ) were purchased from Thermo Fisher Scientific (USA).
Article Title: Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector
Article Snippet: N2B27 medium consisted of DMEM/Nutrient Mixture F-12 Ham (Nacalai Tesque) supplemented with N2 supplement (1×, Thermo Fisher Scientific, Waltham, MA, USA), B27 supplement (1×, Thermo Fisher Scientific), GlutaMAX (1×, Thermo Fisher Scientific), 0.1 mM minimal essential medium non-essential amino acids (MEM NEAA; Thermo Fisher Scientific), and 0.1 mM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA).
Modification:Article Title: Engineering RNA export for measurement and manipulation of living cells
Article Snippet: .. For HEK293, HEK293T, and HEK293FT cells, growth media consisted of Dulbecco’s Modified Eagle Medium (ThemoFisher), 10% fetal bovine serum (ThermoFisher), 100 units/mL penicillin, 100 μM streptomycin, 2 mM L-glutamine (ThermoFisher), 1 mM sodium pyruvate (ThermoFisher), and 1X Minimal Essential Medium Non-Essential Amino Acids (ThermoFisher). .. For K562 and Jurkat cells, growth media consisted of Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement (ThermoFisher), 10% fetal bovine serum (ThermoFisher), 100 units/mL penicillin, 100 μM streptomycin, and 1 mM sodium pyruvate (ThermoFisher).
Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1
Cell Culture:
Virus:Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1
Recombinant:Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1
Saline:Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1
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