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non-essential amino acids solution minimal essential medium (mem neaa) (100  (Thermo Fisher)


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    Thermo Fisher non-essential amino acids solution minimal essential medium (mem neaa) (100
    Non Essential Amino Acids Solution Minimal Essential Medium (Mem Neaa) (100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimal+essential+medium+non-essential+amino+acids+(mem-neaa/pmc11769752-56-6-11
    Average 90 stars, based on 1 article reviews
    non-essential amino acids solution minimal essential medium (mem neaa) (100 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: A 3D"Sandwich" Co-culture System with hPPSCs and hUVECs Supports Mouse Embryo Development from E3.5 to E7.5 In Vitro
    Article Snippet: IVCM was composed of 25% Dulbecco's modi ed eagle medium (DMEM) with high glucose (v/v) (Adamas Life, C8013) supplemented with 75% heat-inactivated (HI) horse serum (v/v) (Gibco, 26050088), 11 mM HEPES (Solarbio, H1095), 100 × GlutaMax (Gibco, 35050-061), 100 × Penicillin Streptomycin (Gibco, 15140-122), 100 × Minimal Essential Medium (MEM) Non-essential Amino Acids (NEAA) (Gibco, 11140-050), 55 μM 2-mercaptoethanol (Gibco, 21985-023), 100 × ITS-X (Gibco, 51500-056), 8 nM β-estradiol (Sigma, HY-B0141), 200 ng/ml progesterone (Sigma, HY-N0437), 25 μM Nacetyl-L-cysteine (Sigma, HY-B0215), 0.25 × N 2 (Gibco, 17502048), 0.5 × B 27 (Gibco, 17504044).

    Article Title: Loading of Extracellular Vesicles with Nucleic Acids via Hybridization with Non-Lamellar Liquid Crystalline Lipid Nanoparticles.
    Article Snippet: Dulbecco’s modified eagle medium (DMEM), phenol red-free DMEM, fetal bovine serum (FBS), GlutaMAX supplement, penicillin-streptomycin (10 000 U mL−1), minimal essential medium (MEM) non-essential amino acids (NEAA) solution (100×), Opti-MEM, trypsin-EDTA (0.25%), UltraPure DNase/RNase-free distilled water, UltraPure agarose, phosphate buffered saline (PBS), Dulbecco’s phosphate buffered saline (DPBS), HEPES 1 M solution, ethanol absolute, Hoechst 33342 and blasticidin S hydrochloride (from Streptomyces Griseochromogenes) were purchased from Thermo Fisher Scientific (USA).

    Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency
    Article Snippet: For the evaluation of naive EPI and PE-like populations in Figs. , and Supplementary Fig. , cells were maintained for at least 3 passages in knockout DMEM (Gibco), 20% knockout serum replacement (KOSR), 2 mM L-glutamine, 1x minimal essential medium non-essential amino acids (Gibco), 1x penicillin/streptomycin (Gibco), 100 μM β-Mercaptoethanol and 1000 U/mL recombinant murine LIF.

    Article Title: Loading of extracellular vesicles with nucleic acids via hybridization with sponge-like lipid nanoparticles
    Article Snippet: Dulbecco’s modified eagle medium (DMEM), phenol red-free DMEM, fetal bovine serum (FBS), GlutaMAX TM supplement, penicillin-streptomycin (10,000 U/mL), minimal essential medium (MEM) non-essential amino acids (NEAA) solution (100x), Opti-MEM TM , trypsin-EDTA (0.25%), UltraPure TM DNase/RNase-free distilled water, UltraPure TM agarose, phosphate buffered saline (PBS), Dulbecco’s phosphate buffered saline (DPBS), HEPES 1 M solution, ethanol absolute, Hoechst 33342 and blasticidin S hydrochloride (from Streptomyces Griseochromogenes ) were purchased from Thermo Fisher Scientific (USA).

    Article Title: Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector
    Article Snippet: N2B27 medium consisted of DMEM/Nutrient Mixture F-12 Ham (Nacalai Tesque) supplemented with N2 supplement (1×, Thermo Fisher Scientific, Waltham, MA, USA), B27 supplement (1×, Thermo Fisher Scientific), GlutaMAX (1×, Thermo Fisher Scientific), 0.1 mM minimal essential medium non-essential amino acids (MEM NEAA; Thermo Fisher Scientific), and 0.1 mM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, USA).

    Modification:

    Article Title: Engineering RNA export for measurement and manipulation of living cells
    Article Snippet: .. For HEK293, HEK293T, and HEK293FT cells, growth media consisted of Dulbecco’s Modified Eagle Medium (ThemoFisher), 10% fetal bovine serum (ThermoFisher), 100 units/mL penicillin, 100 μM streptomycin, 2 mM L-glutamine (ThermoFisher), 1 mM sodium pyruvate (ThermoFisher), and 1X Minimal Essential Medium Non-Essential Amino Acids (ThermoFisher). .. For K562 and Jurkat cells, growth media consisted of Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement (ThermoFisher), 10% fetal bovine serum (ThermoFisher), 100 units/mL penicillin, 100 μM streptomycin, and 1 mM sodium pyruvate (ThermoFisher).

    Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1

    Cell Culture:

    Article Title: Metabolic reprogramming is implicated in the differential response of the CAL-1 plasmacytoid dendritic cell line to autophagy inhibitors.
    Article Snippet: Plasmacytoid dendritic cells (pDCs) produce large amounts of type I Interferons (IFN) and pro-inflammatory cytokines, playing crucial roles in antiviral and anticancer immunity as well as in autoimmune diseases.. Understanding the mechanisms that regulate pDC function is therefore essential.. Autophagy, a process responsible for recycling intracellular components, influences cellular metabolism and modulates immune responses.

    Virus:

    Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1

    Recombinant:

    Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1

    Saline:

    Article Title: Synthetic protein circuits for programmable control of mammalian cell death.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Stable Competent E. coli (High Efficiency) New England Biolabs Cat# C3040H 10-beta Competent E. coli (High Efficiency) New England Biolabs Cat# C3019H Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium ThermoFisher Cat# 11960-069 Roswell Park Memorial Institute (RPMI) 1640 Medium with GlutaMAX supplement, HEPES Gibco Cat# 72400-047 Fetal bovine serum (FBS) Avantor Cat# 97068-085 Penicillin-Streptomycin-Glutamine ThermoFisher Cat# 10378-016 Sodium pyruvate ThermoFisher Cat# 11360070 Minimal Essential Medium Non-Essential Amino Acids ThermoFisher Cat# 11140050 Trypsin-EDTA (0.05%) ThermoFisher Cat# 25300054 Trypsin-EDTA (0.25%) ThermoFisher Cat# 25200056 Dulbecco’s Phosphate Buffered Saline (DPBS) containing calcium and magnesium ThermoFisher Cat# 14040117 Hank’s Balance Salt Solution (HBSS) Gibco Cat# 14025134 Bovine serum albumin (BSA) Sigma Aldrich Cat# A4503 Calcium chloride J.T. .. Baker Cat# 1311-01 Opti-MEM I Reduced Serum Medium ThermoFisher Cat# 31985-070 Carbenicillin, disodium salt Invitrogen Cat# 10177-012 Kanamycin Monosulfate Goldbio Cat# K-120-100 Trypsin Promega Cat# VA9000 Q-VD-OPh Cayman Chemical Cat# 15260 Poly-D-Lysine ThermoFisher Cat# A3890401 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 Hygromycin B Gold InvivoGen Cat# ANT-HG-1 Doxycycline Hydrochloride Sigma Aldrich Cat# D3072 Lipofectamine 2000 ThermoFisher Cat# 11668019 Lipofectamine 3000 ThermoFisher Cat# L3000008 FuGENE HD Promega Cat# E2311 Lipofectamine MessengerMAX ThermoFisher Cat# LMRNA001 Triton X-100 Fisher Scientific Cat# AC327371000 Sytox Green Ready Flow Invitrogen Cat# R37168 Annexin V Pacific Blue Ready Flow Invitrogen Cat# R37177 Annexin V Alexa Fluor 488 Ready Flow Invitrogen Cat# R37174 TO-PRO-3 Iodide Invitrogen Cat# T3605 Trypan Blue Stain, 0.4% Invitrogen Cat# T10282 Vybrant DiD Invitrogen Cat# V22887 LB Broth with agar (Lennox) Sigma Aldrich Cat# L2897-250G Terrific Broth Powder Research Products International Cat# T15000 50x TAE buffer Fisher Scientific Cat# 50-146-818 Agarose I (Molecular Biology Grade) Fisher Scientific Cat# 17852 Blue gel loading dye New England Biolabs Cat# B7021S SyBrSafe Fisher Scientific Cat# S33102 (Continued on next page) Cell 187, 1–16.e1–e9, May 23, 2024 e1



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